Parkin is associated with actin filaments in neuronal and nonneural cells
Identifieur interne : 003460 ( Main/Exploration ); précédent : 003459; suivant : 003461Parkin is associated with actin filaments in neuronal and nonneural cells
Auteurs : Duong P. Huynh [États-Unis] ; Daniel R. Scoles [États-Unis] ; Trang H. Ho [États-Unis] ; Marc R. Del Bigio [Canada] ; Stefan-M. Pulst [États-Unis]Source :
- Annals of Neurology [ 0364-5134 ] ; 2000-11.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Homme.
English descriptors
- KwdEn :
Abstract
Inactivating mutations of the gene encoding parkin are responsible for autosomal recessive juvenile parkinsonism (AR‐JP). However, little information is known about the function and distribution of parkin. We generated antibodies to two different peptides of parkin. By Western blot analysis and immunohistochemistry, we found that parkin is a 50‐kd protein that is expressed in neuronal processes and cytoplasm of selected neurons in the basal ganglia, midbrain, cerebellum, and cerebral cortex. Unlike ubiquitin and α‐synuclein, parkin labeling was not found in Lewy bodies of four sporadic Parkinson disease brains. Parkin was colocalized with actin filaments but not with microtubules in COS1 kidney cells and nerve growth factor–induced PC12 neurons. These results point to the importance of the cytoskeleton and associated proteins in neurodegeneration. Ann Neurol 2000;48:737–744
Url:
DOI: 10.1002/1531-8249(200011)48:5<737::AID-ANA7>3.0.CO;2-D
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001F51
- to stream Istex, to step Curation: 001F51
- to stream Istex, to step Checkpoint: 001181
- to stream Main, to step Merge: 003988
- to stream PascalFrancis, to step Corpus: 000C57
- to stream PascalFrancis, to step Curation: 000066
- to stream PascalFrancis, to step Checkpoint: 000B67
- to stream Main, to step Merge: 003B01
- to stream Main, to step Curation: 003460
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Parkin is associated with actin filaments in neuronal and nonneural cells</title><author><name sortKey="Huynh, Duong P" sort="Huynh, Duong P" uniqKey="Huynh D" first="Duong P." last="Huynh">Duong P. Huynh</name></author><author><name sortKey="Scoles, Daniel R" sort="Scoles, Daniel R" uniqKey="Scoles D" first="Daniel R." last="Scoles">Daniel R. Scoles</name></author><author><name sortKey="Ho, Trang H" sort="Ho, Trang H" uniqKey="Ho T" first="Trang H." last="Ho">Trang H. Ho</name></author><author><name sortKey="Del Bigio, Marc R" sort="Del Bigio, Marc R" uniqKey="Del Bigio M" first="Marc R." last="Del Bigio">Marc R. Del Bigio</name></author><author><name sortKey="Pulst, Stefan" sort="Pulst, Stefan" uniqKey="Pulst S" first="Stefan-M." last="Pulst">Stefan-M. Pulst</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:2D52372958A03FD903531DD0194D41E751F845A1</idno><date when="2000" year="2000">2000</date><idno type="doi">10.1002/1531-8249(200011)48:5<737::AID-ANA7>3.0.CO;2-D</idno><idno type="url">https://api-v5.istex.fr/document/2D52372958A03FD903531DD0194D41E751F845A1/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001F51</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001F51</idno><idno type="wicri:Area/Istex/Curation">001F51</idno><idno type="wicri:Area/Istex/Checkpoint">001181</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001181</idno><idno type="wicri:doubleKey">0364-5134:2000:Huynh D:parkin:is:associated</idno><idno type="wicri:Area/Main/Merge">003988</idno><idno type="wicri:source">INIST</idno><idno type="RBID">Pascal:01-0016490</idno><idno type="wicri:Area/PascalFrancis/Corpus">000C57</idno><idno type="wicri:Area/PascalFrancis/Curation">000066</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000B67</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000B67</idno><idno type="wicri:doubleKey">0364-5134:2000:Huynh D:parkin:is:associated</idno><idno type="wicri:Area/Main/Merge">003B01</idno><idno type="wicri:Area/Main/Curation">003460</idno><idno type="wicri:Area/Main/Exploration">003460</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Parkin is associated with actin filaments in neuronal and nonneural cells</title><author><name sortKey="Huynh, Duong P" sort="Huynh, Duong P" uniqKey="Huynh D" first="Duong P." last="Huynh">Duong P. Huynh</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Rose Moss Laboratory for Parkinson's Disease and Neurodegenerative Disorders, CSMC Burns and Allen Research Institute, Cedars‐Sinai Medical Center, School of Medicine, University of California at Los Angeles, Los Angeles</wicri:cityArea></affiliation></author><author><name sortKey="Scoles, Daniel R" sort="Scoles, Daniel R" uniqKey="Scoles D" first="Daniel R." last="Scoles">Daniel R. Scoles</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Rose Moss Laboratory for Parkinson's Disease and Neurodegenerative Disorders, CSMC Burns and Allen Research Institute, Cedars‐Sinai Medical Center, School of Medicine, University of California at Los Angeles, Los Angeles</wicri:cityArea></affiliation></author><author><name sortKey="Ho, Trang H" sort="Ho, Trang H" uniqKey="Ho T" first="Trang H." last="Ho">Trang H. Ho</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Rose Moss Laboratory for Parkinson's Disease and Neurodegenerative Disorders, CSMC Burns and Allen Research Institute, Cedars‐Sinai Medical Center, School of Medicine, University of California at Los Angeles, Los Angeles</wicri:cityArea></affiliation></author><author><name sortKey="Del Bigio, Marc R" sort="Del Bigio, Marc R" uniqKey="Del Bigio M" first="Marc R." last="Del Bigio">Marc R. Del Bigio</name><affiliation wicri:level="4"><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Pathology, Health Sciences Centre and University of Manitoba, Manitoba Winnipeg</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Pulst, Stefan" sort="Pulst, Stefan" uniqKey="Pulst S" first="Stefan-M." last="Pulst">Stefan-M. Pulst</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Rose Moss Laboratory for Parkinson's Disease and Neurodegenerative Disorders, CSMC Burns and Allen Research Institute, Cedars‐Sinai Medical Center, School of Medicine, University of California at Los Angeles, Los Angeles</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Division of Neurology, Cedars‐Sinai Medical Center, School of Medicine, University of California at Los Angeles, Los Angeles</wicri:cityArea></affiliation><affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Annals of Neurology</title><title level="j" type="abbrev">Ann Neurol.</title><idno type="ISSN">0364-5134</idno><idno type="eISSN">1531-8249</idno><imprint><publisher>John Wiley & Sons, Inc.</publisher><pubPlace>New York</pubPlace><date type="published" when="2000-11">2000-11</date><biblScope unit="volume">48</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="737">737</biblScope><biblScope unit="page" to="744">744</biblScope></imprint><idno type="ISSN">0364-5134</idno></series><idno type="istex">2D52372958A03FD903531DD0194D41E751F845A1</idno><idno type="DOI">10.1002/1531-8249(200011)48:5<737::AID-ANA7>3.0.CO;2-D</idno><idno type="ArticleID">ANA7</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0364-5134</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cytoskeleton</term><term>Human</term><term>Immunofluorescence</term><term>Immunohistochemistry</term><term>Parkinson disease</term><term>Pathophysiology</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Cytosquelette</term><term>Homme</term><term>Immunofluorescence</term><term>Immunohistochimie</term><term>Parkine</term><term>Parkinson maladie</term><term>Physiopathologie</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Inactivating mutations of the gene encoding parkin are responsible for autosomal recessive juvenile parkinsonism (AR‐JP). However, little information is known about the function and distribution of parkin. We generated antibodies to two different peptides of parkin. By Western blot analysis and immunohistochemistry, we found that parkin is a 50‐kd protein that is expressed in neuronal processes and cytoplasm of selected neurons in the basal ganglia, midbrain, cerebellum, and cerebral cortex. Unlike ubiquitin and α‐synuclein, parkin labeling was not found in Lewy bodies of four sporadic Parkinson disease brains. Parkin was colocalized with actin filaments but not with microtubules in COS1 kidney cells and nerve growth factor–induced PC12 neurons. These results point to the importance of the cytoskeleton and associated proteins in neurodegeneration. Ann Neurol 2000;48:737–744</div></front></TEI><affiliations><list><country><li>Canada</li><li>États-Unis</li></country><region><li>Californie</li><li>Manitoba</li></region><settlement><li>Winnipeg</li></settlement><orgName><li>Université du Manitoba</li></orgName></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Huynh, Duong P" sort="Huynh, Duong P" uniqKey="Huynh D" first="Duong P." last="Huynh">Duong P. Huynh</name></region><name sortKey="Ho, Trang H" sort="Ho, Trang H" uniqKey="Ho T" first="Trang H." last="Ho">Trang H. Ho</name><name sortKey="Pulst, Stefan" sort="Pulst, Stefan" uniqKey="Pulst S" first="Stefan-M." last="Pulst">Stefan-M. Pulst</name><name sortKey="Pulst, Stefan" sort="Pulst, Stefan" uniqKey="Pulst S" first="Stefan-M." last="Pulst">Stefan-M. Pulst</name><name sortKey="Scoles, Daniel R" sort="Scoles, Daniel R" uniqKey="Scoles D" first="Daniel R." last="Scoles">Daniel R. Scoles</name></country><country name="Canada"><region name="Manitoba"><name sortKey="Del Bigio, Marc R" sort="Del Bigio, Marc R" uniqKey="Del Bigio M" first="Marc R." last="Del Bigio">Marc R. Del Bigio</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Canada/explor/ParkinsonCanadaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003460 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003460 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Canada
|area= ParkinsonCanadaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:2D52372958A03FD903531DD0194D41E751F845A1
|texte= Parkin is associated with actin filaments in neuronal and nonneural cells
}}
| This area was generated with Dilib version V0.6.29. |  |